SIBER software runs with C and N isotopic data showed spatio-temporal isotopic variations at community, interspecific and intraspecific level.
SIAR results with these new custom values gave more coherent values highlighting the major importance of TEFs values for mixing models data interpretation.Second is a simple question: are the seasonal isotopic composition variations observed for many seagrass detritus macrofauna species due to actual diet changes, or only to isotopic baseline shift of the food sources? Macrofauna and all potential food sources were sampled near STARESO Oceanographic Station (Corsica, 8☄5’E 42☃5’N) in 2011-2012 at each season at two different sites. Animal source showed carnivorous TEFs values while seagrass and algae source showed typical detritivorous values. TEFs calculations showed to be interesting because TEFs seem not to depend on the natural feeding type of the invertebrate but more on the type of food source. Animal food source showed to be the most effectively assimilated with a fast turnover rate while seagrass and algae showed very slow assimilation. Thus, the impact of 3 very different food sources (amphipod powder, algae power, seagrass powder) on the turnover rate of C and N isotopic compositions was tested, and afterwards TEFs for C and N for each source were calculated. This experimental study was planned after a strange result of the SAIR mixing model, giving results opposed to all observations and knowledge we had about this species. More recently… the use of mixing models has exponentially increased to give a more specific vision of organism’s diets and trophic relationships.Two case studies will be presented to give a summary of what’s been done in Liège Oceanology Lab to improve our interpretation of stable isotopes results.First is an experimental calculation of the Trophic Enrichment Factors (TEFs) for one dominant detritivorous species of Mediterranean amphipod inhabiting seagrass detritus: Gammarus aequicauda (Martynov, 1931). Nitrogen, carbon and sulfur stable isotopes are very powerful tools for trophic ecologists to delineate food webs of various ecosystems.
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